FEEDING PATTERN FOR GREATER AMBERJACK Seriola dumerili: EFFECTS ON GROWTH, FEED UTILIZATION AND WELFARE INDICATORS. Barcelona, January 2017
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1 FEEDING PTTERN FOR GRETER MERJCK Seriola dumerili: EFFECTS ON GROWTH, FEED UTILIZTION ND WELFRE INDICTORS arcelona, January 2017
2 Task 21.2 Development of feeding methods. Test of different feeding methods including estimation of daily rhythm and frequency. ction (FCPCT) Definition of feeding pattern for 5 g fish reared in 500 l- tanks for 4 months. Monitoring include: - Growth performance - Feed efficiency - k index - Juvenile quality - Haematological analysis - Histological analysis - iochemical analysis - Immunological analysis. This ction will contribute to deliverable D21.1 Definition of optimum feeding methods for greater amberjack grow out. arcelona, January 2017
3 Not so much references available for the species Feeding rate (%.W./day) Feeding frequency (meals per day) Initial.W. species Satiation S. lalandi bbink % 2 30 S. dumerili Hirazawa et al., % 3 2 S. dumerili Sato et al % 2 50 S. dumerili Takakuwa eyt al., % 3 8 S. lalandi Palstra et al., 2015 Satiation - 100g S. dumerili Yilmaz et al Satiation (around 3.5) 3 20g S. dumerili Fernandez-Montero (submitted) arcelona, January 2017
4 The objec)ve of the present study was to determine the op)mum feeding regime for greater amberjack to op)mize growth, feed u)liza)on and welfare status. arcelona, January 2017
5 Feeding rate (%.W./day) TRETMENTS Feeding frequency (meals per day) treatment pparent satiety 3 T1- S3 pparent satiety 1 T2- S T3-3.5/ T4-3.5/ T5 3.5/ T6 2.5/ T7 2.5/ T8 2.5/1 arcelona, January 2017
6 EXPERIMENTL CONDITIONS Initial weight 20.1 ± 1.8g Number fish /tank 25 fish/tank Tanks 500 l X 24 (3 tanks/treatment) Europa 22 52/20 Dissolved oxygen 7.2 ± 1 mg/l Temperature 21.1 ± 1.44ºC Experimental period 120 days arcelona, January 2017
7 Samplings Monthly iological parameters Parameters Weight gain FCPC T FCR Final sampling (120 days) iological Parameters Weight gain FCR Immunology Serum lysozyme activity Serum peroxidase activity lood biochemistry LP, Lipase, Cholesterol, Glucose, Triglycerides arcelona, January 2017
8 iochemistry Proximal composition - Crude protein - Crude Lipids - Whole fish - Feeds - Nutrient retention Histology Liver intestine arcelona, January 2017
9 Results arcelona, January 2017
10 250 ody Weight (g) S3 s1 3.5/3 3.5/4 3.5/1 2.5/3 2.5/4 2.5/1 Different letters denote significant P<0.05) differences arcelona, January 2017
11 Two way NOV P- value Feeding Rate Feeding frequency Rate x Frequency Two-way NOV analyses showed that fish final weight was affected (P<0.05) by the amount of fish provided and not by the number of meals. esides, there was a significant (P<0.05) interaction between both factors. arcelona, January 2017
12 Length 19 cm S3 s1 3.5/3 3.5/4 3.5/1 2.5/3 2.5/4 2.5/ K Factor Different letters denote significant P<0.05) differences 0.00 arcelona, January 2017 S3 s1 3.5/3 3.5/4 3.5/1 2.5/3 2.5/4 2.5/1
13 SGR SGR Two way NOV P- value Feeding Rate Feeding frequency S3 s1 3.5/3 3.5/4 3.5/1 2.5/3 2.5/4 2.5/1 Rate x Frequency arcelona, January 2017 Different letters denote significant P<0.05) differences
14 FCR FCR S3 s1 3.5/3 3.5/4 3.5/1 2.5/3 2.5/4 2.5/1 Two way NOV P- value Feeding Rate Feeding frequency Rate x Frequency arcelona, January 2017 Different letters denote significant P<0.05) differences
15 Feed intake (g dm/fish) Intake Two way NOV P- value Feeding Rate Feeding frequency S3 s1 3.5/3 3.5/4 3.5/1 2.5/3 2.5/4 2.5/1 Rate x Frequency arcelona, January 2017 Different letters denote significant P<0.05) differences
16 Protein Efficiency Ratio PER Two way NOV P- value Feeding Rate Feeding frequency Rate x Frequency S3 s1 3.5/3 3.5/4 3.5/1 2.5/3 2.5/4 2.5/1 Different letters denote significant P<0.05) differences
17 Proximal composition Protein Lipids % (D.W.) S3 s1 3.5/3 3.5/4 3.5/1 2.5/3 2.5/4 2.5/1 arcelona, January 2017
18 FCPCT Histological analysis S3 3.5/3 3.5/4 S1 3.5/1 2.5 Currently in progress arcelona, January 2017
19 Protein Retention Protein Reten5on S3 s1 3.5/3 3.5/4 3.5/1 2.5/3 2.5/4 2.5/1 Two way NOV P- value Feeding Rate Feeding frequency Rate x Frequency arcelona, January 2017 Different letters denote significant P<0.05) differences
20 lood biochemical parameters arcelona, January 2017
21 lood biochemical parameters lkaline Phosphatase LP Lipase Different letters denote significant P<0.05) differences arcelona, January 2017
22 Serum Lysozyme activity Different letters denote significant P<0.05) differences arcelona, January 2017
23 Peroxidase activity arcelona, January 2017
24 Preliminary Conclusions feeding ratio of 3.5% body weight per day produced higher growth, best FCR, higher protein retention Increasing meals per day up to 3 induced better growth, FCR and protein retention. No differences were found increasing meals per day up to 4. feeding ratio of 3.5% body weight per day, in 3 meals per day is the best protocol for Greater amberjack juveniles. Offering only one meal per day increases some blood biochemical parameters that suggest lipidic metabolism imbalance. arcelona, January 2017
25 Thank you for your attention International Symposium on Fish Nutrition and Feeding (ISFNF2018) June 2018 arcelona, January 2017
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