IRIC's Core Facility Pricing Updated July 11th, 2014

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1 IRIC's Core Facility Pricing Updated July 11th, 2014 For information, please contact BIO-IMAGING - rate per hour Confocal $ $ $ $ Axio-Imager and others $ $ $ $ BIO-INFORMATICS - rate per hour Bioinformatician $ $ $ $ FLOW CYTOMETRY - rate per hour Analysis BD LSR II, self operator $ $ $ $ BD LSR II, with assistance $ $ $ $ BD LSR II with High troughput sampler, self operator $ $ $ $ BD LSR II with High troughput sampler, with assistance $ $ $ $ BD FACSCanto II, self operator $ $ $ $ BD FACSCanto II, with assistance $ $ $ $ ique screener, self operator $ $ $ $ ique screener, with assistance $ $ $ $ Sorting BD FACS Aria $ $ $ $ AutoMacs (per sample) $ $ $ $ Data analysis or Consultation $ $ $ $ HIGH THROUGHPUT SCREENING Plate reader (per hour) Envision (Perkin-Elmer) 40 $ 40 $ 60 $ 100 $ SpectraMax (Molecular Devices) 40 $ 40 $ 60 $ 100 $ Synergy Neo (Biotek) 40 $ 40 $ 60 $ 100 $ Operetta (Perkin-Elmer) 10 $ 10 $ 20 $ 40 $ Biomek FX (Beckman) 100 $ 100 $ 140 $ 200 $ Integrated robotic system (per hour) 100 $ 100 $ 140 $ 200 $ Technical assistance, data analysis & consultancy (per hour) 45 $ 45 $ 60 $ 130 $ Page 1 de 10

2 GENOMICS - rate per sample Sanger sequencing Sequencing reactions 7.40 $ 7.90 $ 8.40 $ $ Sequencing reactions (>384 samples) 5.30 $ 6.30 $ 7.40 $ $ Sequencing - BAC extremities $ $ $ $ ABI 3730 sequencer plate read - 96-well $ $ $ $ qpcr - gene expression analysis qpcr assay with UPL probe (design and validation) $ $ $ $ Reverse transcription - cdna synthesis 7.20 $ 7.69 $ 8.18 $ $ Reverse transcription - cdna synthesis > $ 6.41 $ 6.82 $ $ Reverse transcription - cdna synthesis > $ 5.24 $ 5.57 $ 8.38 $ DNAse treatment of RNA sample 3.20 $ 3.70 $ 4.20 $ 5.30 $ qpcr reaction with UPL probe 1.56 $ 1.67 $ 1.77 $ 2.67 $ qpcr reaction with UPL probe > $ 1.38 $ 1.46 $ 2.20 $ qpcr reaction with SYBR green 1.15 $ 1.23 $ 1.30 $ 1.96 $ qpcr reaction - Low Density Array (excluding array) 0.36 $ 0.39 $ 0.41 $ 0.62 $ qpcr plate read wells $ $ $ $ cdna pre-amplification $ $ $ $ Bioanalyzer - sample quality control Bioanalyzer RNA Nano (1 to 12 samples) $ $ $ $ Bioanalyzer RNA Pico (1 to 11 samples) $ $ $ $ Bioanalyzer DNA (1 to 12 samples) $ $ $ $ Bioanalyzer DNA High Sensitivity (1 to 11 samples) $ $ $ $ Next-generation sequencing (Illumina HiSeq 2000) Sequencing on the HiSeq2000, 200 cycles Sequencing on the MiSeq, 50 cycles Transcriptome (RNA seq) Genome (DNA seq) Exome Genomic regions of interest Small RNAs Quality control of libraries Contact us for a quote : Page 2 de 10

3 HISTOLOGY Paraffin Processing and embedding (per block) 2.84 $ 3.90 $ 5.60 $ 6.50 $ Decalcification (per cassette) 1.86 $ 2.24 $ 2.99 $ 3.50 $ Cassetting (per cassette) 2.07 $ 2.27 $ 2.50 $ 3.50 $ Microtome sections (per slide) 1.73 $ 2.40 $ 3.20 $ 3.70 $ Step sections (per slide) 1.94 $ 2.61 $ 3.28 $ 4.00 $ Additional sections (per section) 0.43 $ 0.68 $ 0.92 $ 2.50 $ H&E (per slide) 2.20 $ 2.41 $ 3.00 $ 3.97 $ HPS (per slide) 2.51 $ 2.71 $ 2.92 $ 4.09 $ FFPE complete service: processing, embedding, 1 section, 1 H&E (per block) 5.64 $ 7.82 $ 9.99 $ $ Frozen Tissue Freezing/OCT (per block) 2.35 $ 3.00 $ 3.25 $ 4.50 $ Cryostat sections (per slide) 2.73 $ 3.72 $ 4.72 $ 5.95 $ H&E (per slide) 1.31 $ 1.81 $ 2.31 $ 2.92 $ Frozen complete service: OCT, 1 section, 1 H&E (per block) 5.31 $ 7.37 $ 9.43 $ $ Histological stains (per slide) (other stains available upon request) Toluidine blue 3.76 $ 4.13 $ 4.96 $ 6.95 $ Gram 7.94 $ 8.74 $ $ $ Groccot 5.69 $ 6.26 $ 7.51 $ $ May Grunwald Giemsa 6.32 $ 6.95 $ 8.34 $ $ Movatt's 7.94 $ 8.74 $ $ $ Nuclear Fast Red 3.16 $ 3.48 $ 4.50 $ 5.84 $ PAS 4.27 $ 4.70 $ 6.50 $ 8.00 $ PAS - Alcian Blue 6.32 $ 6.95 $ 8.34 $ $ Sirius red 5.81 $ 6.39 $ 7.67 $ $ Masson's Trichrome 6.92 $ 7.61 $ 9.13 $ $ Von Kossa 7.60 $ 8.36 $ $ $ IHC/IF (per slide) (Biomarkers list available upon request) IHC without Iry antibody $ $ $ $ IF (TxRed or FITC) without Iry antibody $ $ $ $ IHC multimer without Iry antibody $ $ $ $ Dual stain without Iry antibody $ $ $ $ Consultation for antibody optimization (per hour) $ $ $ $ Tissue Microarray (TMA) TMA tissue plug (per core) 3.69 $ 5.13 $ 6.40 $ 9.18 $ TMA sections (per TMA) 3.18 $ 4.43 $ 5.55 $ 7.96 $ Page 3 de 10

4 HISTOLOGY Imaging - Analysis Scan - 20X (per slide) 4.31 $ 6.46 $ 7.60 $ 9.72 $ Scan - 40X (per slide) 9.72 $ $ $ $ Scan - 20X Fluo (per slide) 4.47 $ 6.83 $ 8.35 $ $ Scan - 40X Fluo (per slide) 9.72 $ $ $ $ Data storage (per GO/3 months) 1.10 $ 1.63 $ 2.15 $ 5.41 $ Image analysis protocol setup (per hour) $ $ $ $ Quantitative analysis 6.99 $ $ $ $ LMD usage without assistance (per hour) $ $ $ $ Fluorescence microscope usage / photographs without assistance (per hour) $ $ $ $ Analysis by the pathologist (per hour) $ $ $ $ Other Services Technical assistance (per hour) $ $ $ $ Miscellaneous DVD (per DVD) 0.85 $ 0.85 $ 0.85 $ 1.11 $ LMD membrane slides (per slide) 5.06 $ 5.06 $ 5.06 $ 6.58 $ LMD collection caps (per tube) 4.28 $ 4.28 $ 4.28 $ 5.56 $ Slide box X30 (per box) 1.76 $ 1.76 $ 1.76 $ 2.29 $ Slide box X100 (per box) 3.69 $ 3.69 $ 3.69 $ 4.80 $ Micro - cassettes (per cassette) 1.45 $ 1.45 $ 1.45 $ 1.89 $ Macro - cassettes (per cassette) 0.40 $ 0.40 $ 0.40 $ 0.52 $ Pad (per pad) 0.27 $ 0.27 $ 0.27 $ 0.35 $ Blades for trimming (per blade) 2.10 $ 2.10 $ 2.10 $ 2.73 $ Page 4 de 10

5 NMR-BIOPHYSICS - rate per hour NMR Sample preparation $ $ $ $ 600 MHz Cold Probe - set up $ $ $ $ 600 MHz Cold Probe - long runs 2.35 $ 2.50 $ 2.60 $ $ 600 MHz Cold Probe - short runs (<24 hours) $ $ $ $ 600 MHz Cold Probe - automated procedures $ $ $ $ Data processing $ $ $ $ Data analysis* $ $ $ $ Training - NMR $ $ $ $ Training - analysis, calculations $ $ $ $ Analytical Ultra-Centrifuge (AUC) Start up fee $ $ $ $ Assembly/cleaning $ $ $ $ Sedimentation velocity (~10 hours) $ $ $ $ Sedimentation equilibrium - flat rate (~24-48 hours) $ $ $ 1,260 $ Data analysis $ $ $ $ Circular Dichroism (CD) Run and basic analysis $ $ $ $ Data analysis $ $ $ $ Training - CD $ $ $ $ Fluorescence Run and basic analysis $ $ $ $ Training - Fluorescence $ $ $ $ Biophysics packages Basic - AUC, CD, (NMR), (fluorescence) 655 $ 740 $ 790 $ 2,100 $ Mutant - CD, AUC: altered structure, aggregation state 655 $ 740 $ 790 $ 2,100 $ Interaction - fluorescence, AUC, CD 1,312 $ 1,470 $ 1,580 $ 4,200 $ RNA/DNA - base pairing (NMR), Tm (CD) 1,312 $ 1,470 $ 1,580 $ 4,200 $ Structure - protein expression, labelling, NMR data collection, data analysis and structure determination** 13,135 $ 14,450 $ 15,760 $ 42,000 $ Computational Linux cluster (per hour) NMR analysis - $ - $ - $ 2.10 $ Strucutre calculations - ARIA/CYANA - $ - $ - $ 8.40 $ Strucutre calculations - cns - $ - $ - $ 2.10 $ Visualisation tools Training and analysis $ $ $ $ Movies $ $ $ $ *Fees for avanced analyses are subject to negotiation **Estimate for a 100 residue protein. Actual fee will be determined on a case per case basis Page 5 de 10

6 PROTEOMICS - rate per sample Sample preparation Liquid digestion $ $ $ $ 1D or 2D gel + silver staining (<8 samples) (per gel) $ $ $ $ Destaining (per gel) $ $ $ $ 1D/2D in-gel digestion + peptide extraction $ $ $ $ Phosphopeptide enrichment $ $ $ $ Mass spectrometry LC-MS or LC-MS/MS $ $ $ $ Long run LC-MS/MS $ $ $ $ 2D LC-MS/MS (6 sample fractions) $ $ $ 1,551 $ Exact Mass $ $ $ $ 2D spot analysis et MRM $ $ $ $ Analysis (per hour) $ $ $ $ Scaffold analysis $ $ $ $ Total - liquid digest + LC-MS/MS $ $ $ $ Total - destain, digest, extraction, LC-MS/MS* $ $ $ $ Total - gel, destain, digest, extraction, LC-MS/MS* $ $ $ $ *Price per sample, based on the processing of 8 samples TRANSGENESIS Pronuclear DNA microinjection Microinjection C57BL/6 5,000 $ 5,590 $ 6,429 $ 14,145 $ ES cells injection Blastocysts C57BL/6 3,657 $ 4,715 $ 5,400 $ 12,710 $ Morula CD1 3,657 $ 4,715 $ 5,400 $ 12,710 $ Blastocysts B6(Cg)Tyrc2j/j (albinos B6) 4,536 $ 6,075 $ 7,000 $ 12,710 $ Embryo Cryopreservation Tg x Tg (300 embryos) 1,790 $ 1,950 $ 2,250 $ 5,230 $ Homo x wt C57BL/6 (300 embryos) 2,000 $ 2,300 $ 2,650 $ 5,230 $ Hetero x wt C57BL/6 (600 embryos) 3,300 $ 3,800 $ 4,370 $ 5,230 $ Storage in liquid nitrogen (per strain/month) 4 $ 6 $ 8 $ 10 $ Thawing and reimplantation (cryo procedure performed at IRIC) 660 $ 780 $ 900 $ 2,050 $ Sperm Cryopreservation Cryo and validation by IVF reimplantation 680 $ 795 $ 900 $ 2,155 $ Storage in liquid nitrogen (per strain/month) 4 $ 6 $ 8 $ 10 $ Thawing and reimplantation (cryo procedure performed at IRIC) 1,130 $ 1,330 $ 1,500 $ 3,590 $ In vitro fertilization (Fresh Sperm) FIV C57BL/6 1,130 $ 1,330 $ 1,500 $ 3,590 $ Rederivation Tg x Tg 1,200 $ 1,435 $ 1,650 $ 3,795 $ Homo or Hetero x wt C57BL/6 1,200 $ 1,435 $ 1,650 $ 3,795 $ Sperm - external source (Thawing, IVF et reimplantation) 1,300 $ 1,530 $ 1,600 $ 3,590 $ Embryos - external source (Thawing, IVF et reimplantation) 710 $ 830 $ 950 $ 2,050 $ Page 6 de 10

7 BIOPHARMACY - Pharmacy faculty - Jean-Coutu building ADMET, PK et bioanalysis CYTOGENETICS - HMR ( Contact us for a quote : Mouse cytogenetic services Cells harvesting (suspension cell culture) $ $ $ $ Cells harvesting (adherent cell culture) $ $ $ $ Standard G-banding Karyotyping G-banding preparation $ $ $ $ Search and capture of metaphases $ $ $ $ Analysis of 20 metaphases and karyotyping $ $ $ $ Verification and validation of the karyotypes $ $ $ $ Preparation and print of 5 karyotypes $ $ $ $ Basic price $ $ $ $ * For complex karyotype : see pricing for complex karyotype by SKY Chromosome breakage studies Preparation of different alkylating agents $ $ $ $ Standard Giemsa preparation $ $ $ $ Search and capture of metaphases $ $ $ $ Chromosome breakage count of 50 cells (less than 5 breakages/cell) $ $ $ $ Verification and validation of the breakagestudies $ $ $ $ Preparation and print of images $ $ $ $ Basic price $ $ $ $ Complex breakage $ 1,350 $ 1,720 $ 2,150 $ Giemsa stain and chromosome count G-banding preparation $ $ $ $ Search and capture of metaphases $ $ $ $ Chromosomes counting $ $ $ $ Verification and validation of the analysis 8.75 $ $ $ $ Preparation and print of images $ $ $ $ Basic price $ $ $ $ Page 7 de 10

8 FISH (BACs) BAC probe $ $ $ $ BAC extraction $ $ $ $ BAC labelling $ $ $ $ FISH technique (hybridization, washing, detection) for BAC validation on a control slide $ $ $ $ BAC validation on metaphases (microscopy analysis) 8.75 $ $ $ $ FISH technique (hybridization, washing, detection) for BAC study on the test sample $ $ $ $ Search and capture of metaphases $ $ $ $ Verification and validation of the analysis $ $ $ $ Preparation and print of FISH images $ $ $ $ Final rapport $ $ $ $ Basic price $ $ 1,096 $ 1,370 $ Spectral Karyotyping (SKY) Mouse & Rat SKY technique (hybridization, washing, detection) $ $ $ 1,088 $ Search and capture of metaphases $ $ $ $ Karyotyping and metaphases anlaysing $ $ $ $ Verification and validation of the spectral karyotypes $ $ $ $ Preparation and print of spectral karyotypes (SKY) $ $ $ $ Final report $ $ $ $ Basic price 1,129 $ 1,358 $ 1,578 $ 1,973 $ *Spectral Karyotyping (SKY) Mouse & Rat cell line with complex karyotype 2,490 $ 2,570 $ 3,150 $ 3,938 $ Human Cytogenetics Services Cells harvesting (suspension cell culture) $ $ $ $ Cells harvesting (adherent cell culture) $ $ $ $ Standard G-banding karyotyping G-banding preparation $ $ $ $ Search and capture of metaphases $ $ $ $ Analysis of 20 metaphases and karyotyping $ $ $ $ Verification and validation of the karyotypes $ $ $ $ Preparation and print of 5 karyotypes $ $ $ $ Basic price $ $ $ $ Complex karyotype (G-banding) $ $ 1,060 $ 1,325 $ Page 8 de 10

9 Chromosome breakage studies Preparation of different alkylating agents $ $ $ $ Standard Giemsa preparation $ $ $ $ Search and capture of metaphases $ $ $ $ Chromosome breakage count of 50 cells (less than 5 breakages/cell) $ $ $ $ Verification and validation of the analysis $ $ $ $ Preparation and print of images $ $ $ $ Basic price $ $ $ $ Complex $ 1,350 $ 1,720 $ 2,150 $ Giemsa stain and chromosome count G-banding preparation $ $ $ $ Search and capture of metaphases $ $ $ $ Chromosomes counting $ $ $ $ Verification and validation of the analysis 8.75 $ $ $ $ Preparation and print of images 8.75 $ $ $ $ Basic price $ $ $ $ FISH (commercial probe) Commercial probe $ $ $ $ FISH technique (hybridization, washing, detection) $ $ $ $ Count, analysis, image capture $ $ $ $ Verification and validation of the analysis 8.75 $ $ $ $ Preparation and print of FISH images 8.75 $ $ $ $ Basic price $ $ $ $ FISH (BACs) BAC probe $ $ $ $ BAC extraction $ $ $ $ BAC labelling $ $ $ $ FISH technique (hybridization, washing, detection) for BAC validation on a control slide $ $ $ $ Page 9 de 10

10 BAC validation on metaphases (microscopy analysis) 8.75 $ $ $ $ FISH technique (hybridization, washing, detection) for BAC study on the test sample $ $ $ $ Search and capture of metaphases $ $ $ $ Verification and validation of the analysis $ $ $ $ Preparation and print of FISH images $ $ $ $ Final report $ $ $ $ Basic price $ $ 1,096 $ 1,371 $ Human Cytogenetics Services FISH (Fosmids) Fosmid probe $ $ $ $ Fosmid extraction $ $ $ $ Fosmid labelling $ $ $ $ FISH technique (hybridization, washing, detection) for Fosmid validation on a control slide $ $ $ $ Fosmid validation on metaphases (microscopy analysis) 8.75 $ $ $ $ FISH technique (hybridization, washing, detection) for Fosmid study on the test sample $ $ $ $ Search and capture of metaphases $ $ $ $ Verification and validation of the analysis $ $ $ $ Preparation and print of FISH images $ $ $ $ Final report $ $ $ $ Basic price $ $ 1,096 $ 1,371 $ Spectral karyotyping (SKY) Human quality and mitotic index evaluat $ $ $ $ SKY technique (hybridization, washing, detection) $ $ $ $ Search and capture of metaphases $ $ $ $ Karyotyping and metaphases anlaysis $ $ $ $ Verification and validation of the analysis $ $ $ $ Preparation and print of spectral karyotypes (SKY) $ $ $ $ Final report $ $ $ $ Basic price $ 1,195 $ 1,415 $ 1,769 $ *Spectral Karyotyping (SKY) human sample with complex karyotype 2,100 $ 2,500 $ 2,900 $ 3,625 $ Karyotype analysis by a cytogeneticist (per hour) $ $ $ $ Page 10 de 10